When M2 holds a SATA-type M.2 device, SATA3_1 becomes inactive.
When M2 holds a SATA-type M.2 device, SATA3_1 becomes inactive.
M.2 is a common connector standard. M.2 SSDs may use PCIe (NVMe) or SATA interfaces. The user guide details information beginning on page 35 of the provided PDF: https://download.asrock.com/Manual/B560M Steel Legend.pdf M2_1 is compatible only with NVMe drives. M2_2 supports both NVMe and SATA, but placing a SATA SSD in that slot turns off the SATA3_1 port near the front I/O. To utilize both extended M.2 bays and dual SATA ports, use two NVMe M.2 drives.
Check the connector type on the SSD. If it has a SATA port, it’s SATA; if it uses PCIe or M.2 interface, it’s not SATA.
For SATA M.2 SSDs you frequently encounter mentions of SATA or AHCI, and the specs will indicate speeds below 600 MB/s (roughly 550 MB/s is the upper limit on SATA). As others have mentioned, the M.2 interface is built to handle either NVMe (PCI-E) or SATA-based drives, but when using the SATA version, the connections to the SATA 3.1 port are rerouted to the M.2 slot, rendering that port inaccessible.