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Windows may request a fresh key not used in the past 30 days. Each device gets just one key.
It seems uncertain if the setup works with a bootable USB drive. You might need a different activation key. Windows can be tricky when it comes to activation, especially with old PCs. To clarify: using a USB to install Windows on an existing machine usually works without a new key—just use your already-activated key. If you're installing on another computer, you'll likely need a fresh key since Windows only allows 30 days of use. Booting from the USB may require a new key because Windows treats it as a new device. For certainty, testing it yourself or contacting Microsoft would help.