The CPU or chipset controls which PCIe slot is active.
The CPU or chipset controls which PCIe slot is active.
On your motherboard, certain PCIe ports are managed by the CPU while others depend on the chipset. Your recent change of the Wi-Fi card from the bottom X1 slot to the second X16 slot should not affect future performance. The GPU is functioning well on the X16 port, and Wi-Fi is also working as expected.
The lower 16x slot and single slots probably link to the chipset. The dark port is likely set up for 4x and works alongside the bottom M.2 slot. It’s important to review the manual for details.